Potosí or Potosi may refer to:
Places
- Bolivia
- Colombia
- Jamaica
- Mexico
- Nicaragua
- United States
- Venezuela
Other uses
- Potosi (ship), a German sailing ship named after the Bolivian city
- Potosi Pinyon (Pinus culminicola), a species of pine tree native to northeast Mexico
- Potosi pupfish (Cyprinodon alvarezi), a species of fish once endemic to Mexico, but now extinct in the wild